Journal: Cell Death Discovery

Article Title: Discovery of synthetic G-quadruplex DNA as SARS-CoV-2 helicase inhibitor with antiviral, anti-inflammatory and antioxidative properties

doi: 10.1038/s41420-026-03006-0

Figure Lengend Snippet: A Schematic display of the experimental set-up. B Immunoblot analysis of viral nucleocapsid protein (NP) in the presence of GQ20-PTO. C Densitometric analysis of NP. Signals were normalized to GAPDH and related to the virus control (ctrl). Statistical analysis was performed in relation to virus control using one-way ANOVA with P values indicated in the graph. D Quantitative levels of virus-induced caspase 3/7 activation in the presence of GQ20-PTO. P values of one-way ANOVA are indicated. E Quantitative measurement of the transepithelial electrical resistance (TEER) after virus infection in the presence of GQ20-PTO. P values of one-way ANOVA are indicated in the graph. F Immunoblot analysis of interferon (IFN) signaling of infected and treated ALI cultures. G Densitometric analysis of pSTAT, Mx1 and ISG15 shown in ( F ). P values of one-way ANOVA are indicated in the graph. H Representative images of HBE ALI cultures treated with (1 µM) Cy3-labeled GQ20-PTO (applied apically and to the basal medium) for 24 h; Cy3 in orange, Hoechst 33324 in blue. Scale bar: 50 µm.

Article Snippet: HBE ALI lysates were analyzed with primary antibodies against GAPDH (#2118, 1:4000, CST), ISG15 (#sc-166755,1:200, Santa Cruz Biotechnology), Mx1 (#37849, 1:1000, CST), SARS-CoV-2 Nucleocapsid (#40143-R019, 1:10 000, Sino Biological), STAT1 (#9172, 1:1000, CST), phospho-STAT1 Y701 (#9171, 1:1000, CST).

Techniques: Western Blot, Virus, Control, Activation Assay, Infection, Labeling

Journal: bioRxiv

Article Title: Selective JAK Inhibition Reveals Paradoxical and Hierarchical Control of interferon-γ-driven Autoimmunity in AIRE Deficiency

doi: 10.64898/2026.03.05.709894

Figure Lengend Snippet: Untreated Air e −/− mice and Air e −/− mice treated for four weeks with selective JAK1i, JAK2i, or JAK3i were analyzed. Lungs were processed for intracellular cytokine staining, qPCR, ELISA, and immunoblot analyses. ( A-B ) Representative flow cytometry plots showing IFN-γ production by CD4 + and CD8 + T cells. ( C-D ) Frequency (of total CD4 + and CD8 + T cells) and absolute numbers of IFN-γ + CD4 + and IFN-γ + CD8 + T cells in the lung. ( E-F ) Relative Ifng mRNA expression and IFN-γ protein concentrations in lung homogenates. ( G ) Relative Stat1 mRNA expression. ( H ) Representative immunoblots of phospho-STAT1 (pSTAT1), total STAT1, and GAPDH. ( I-J ) Quantification of total STAT1 and phospho-STAT1 normalized to GAPDH. For IFN- γ + CD4 + and IFN-γ + CD8 + T cells: n = 9-14 mice per group from four independent experiments. For Ifng and Cxcl9 mRNA: n = 15-22 mice per group from four independent experiments. For Stat1 _mRNA: n = 10-17 mice per group from three independent experiments. For CXCL9 protein: n = 5-10 mice per group from two independent experiments. For STAT1 and pSTAT1 immunoblots: n = 15-22 mice per group from four independent experiments). Statistical analyses were performed using one-way ANOVA or Kruskal-Wallis test with Dunn’s multiple comparisons to the untreated Air e −/− mice. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: Primary antibodies against GAPDH (catalog no. 5174S), STAT1 (catalog no. 14995S), and pSTAT1 (catalog no. 9167S) (Cell Signaling Technologies, USA) were used at 1:1,000 dilution, with HRP-conjugated anti-rabbit IgG conjugated with HRP (catalog no.7074S; Cell Signaling Technology) as the secondary antibody.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Expressing